中国水稻科学

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (1): 41-48.DOI: 10.3969/j.issn.1001-7216.2014.01.006

• 研究报告 • 上一篇    下一篇

一个水稻黄绿叶突变体ygl10的遗传分析和基因定位

杨海莲#,刘敏#,郭旻,李荣德,张宏根,严长杰*   

  1. 扬州大学 农学院 江苏省作物遗传生理国家重点实验室培育点/教育部植物功能基因组学重点实验室， 江苏 扬州225009；
  • 收稿日期:2013-07-04 修回日期:2013-07-22 出版日期:2014-01-10 发布日期:2014-01-10
  • 通讯作者: 严长杰*
  • 基金资助:

    国家自然科学基金资助项目（31171158）； 农业部转基因专项（2011ZX08009003005）； 江苏省自然科学基金资助项目（BK2012684）；江苏省六大人才高峰； 江苏高校优势学科建设工程资助项目。

Genetic Analysis and Position Cloning of a yellowgreen leaf 10 (ygl10) Gene, Responsible for Leaf Color in Rice

YANG  Hailian#, LIU Min#, GUO Min, LI Rongde, ZHANG Honggen, YAN Changjie*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou 225009, China；
  • Received:2013-07-04 Revised:2013-07-22 Online:2014-01-10 Published:2014-01-10
  • Contact: YAN Changjie*

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摘要： 以籼稻9311为背景的水稻突变体中发现一个黄绿叶突变体(yellowgreen leaf, ygl10)。形态分析表明，与野生型9311相比，ygl10突变体株高、穗长降低，结实率下降。叶绿素含量测定表明，ygl10突变体中叶绿素a、叶绿素b和类胡萝卜素含量均极显著降低，其中叶绿素b降幅最大，只有野生型的2%。叶绿体超微结构观察表明，突变体中类囊体和基粒片层数量明显减少。遗传分析结果表明，该黄绿叶突变体由一隐性核基因控制。进一步利用分子标记将ygl10定位在水稻第10染色体约380 kb的区段内。对该区段内存在的ORF进行序列分析，发现编码叶绿素a氧化酶（chlorophyll a oxygenase）基因（OsCAO1）的第9个外显子存在5个碱基缺失，从而导致提前出现终止密码子，推测CAO1即为ygl10的候选基因。

关键词: 水稻, 黄绿叶, 分子标记, 基因克隆

Abstract: A rice mutant characterized by yellowgreen leaves (named as ygl10) was obtained from an indica cultivar 9311 via radiation of 60Coγ ray. Morphological analysis showed that, the ygl10 mutant exhibited shorter plant height and panicle length, decreased seed setting rate compared to its wild type (9311). Moreover, the chlorophyll content in the ygl10 mutant leaves dramatically decreased, especially for chlorophyll b content, being only about 2% of that in  its wild type. In addition, the SEM results showed that the numbers of grana lamella, and thylakoids in the mutant were less than those in the wild type. Genetic analysis and gene mapping showed that the yellow green leaf mutant was controlled by a single recessive gene, which  was located  in a 380 kb region on chromosome 10. We further analyzed the putative candidate open reading frames in the target region through sequencing, and the results showed that there was a 5nucleotide deletion in the ninth exon of CAO1 gene (encoding chlorophyll a  oxygenase involved in the biosynthesis of chlorophyll b), resulting in the frame shift to generate a new premature stop codon. Therefore, it is deduced that CAO1 is the candidate of ygl10.

Key words: rice, yellowgreen leaf mutant, molecular marker, gene cloning

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